» 2007 » May

A Day in the Lab

May 31st, 2007

I received a request to share with you all some of the things that are involved with my job as a clinical microbiologist.  With fair warning, this will be boring so you might as well go over and visit Brad instead. 

Here are some pictures of my lovely assistant, Arlene the microbiology queen, setting up a urine culture.

uc 1.JPG

Arlene

uc 2.JPG

Arlene selects two types of agar filled Petri dishes that will support a variety of bacteria known to cause urinary tract infections.  Agar has the consistency of Jello.  She sterilizes a metal loop that is calibrated to deliver 10 micrograms of urine to the surface of the agar.  The red plate contains sheep blood cells to enhance the bacterial growth.  We don't kill the sheep.  They actually have very nice lives on a farm.

 uc 3.JPG

uc 4.JPG

The loop is dipped into the specimen.

uc 5.JPG

And then applied to the surface of the agar.  Careful attention is paid to not slicing up the agar with the wire loop so as not to jeopardize the integrity of the agar.

uc 8.JPG

She sterilizes the loop again,

uc 9.JPG

and innoculates the second plate.  Notice that all specimen manipulation takes place in a biohazard class II safety chamber.  This way we limit exposure to potential pathogenic bacteria.  

uc 10.JPG

The plates are then incubated at 37 degrees C which is the same temperature of the human body.  

uc 11.JPG

uc 12.JPG

Arlene gets cheeky for the camera.  Work it, girl, work it.

uc 13.JPG

The next day, we examine the dishes for growth.

uc 14.JPG

Here is a typical culture.  Each tiny, little dot is a bacterial colony that has about 100,000 bacterial cells each.  That's a lot of bacteria!  The organism is one you may have heard of Staphylococcus aureus and happens to be one of those "super bugs" that you've probably read about.  The big white dot is filter paper disc that has been soaked in a particular antibiotic that helps in identification.  Although it's difficult to see, what appears to be a zone of inhibition, is actually not - which gives us a big clue as to what this bug is.  We identify the organsims by combining colony morphology, microscopic examination, biochemical tests, and antimicrobial susceptibility testing.  

Once the identification and susceptibility testing is completed, we check to see that the antibiotic perscribed will be effective against the infection.  If not, then we inform the physician that he or she will need to make a change in the prescription.  

You're still reading?!?  Man, you must be bored if you made it all the way to the end!  Just wait until you see what we do with sputum.

 

Memorial

May 28th, 2007
deer-in-arlington-barry-scott-november-2005-photo-01.jpg

What a Card

May 21st, 2007

Me:  "Hey Mark.  I've hit a dry spell and I don't have anything interesting to write about.  Say something funny for the blog."

Mark:  "Something funny for the blog."

Me:  "Well, I guess that will have to do." 

Posted in random | 6 Comments »

Insert Foot

May 19th, 2007

So, we were a bit short staffed the other day and I had to go into work early to open the laboratory for the morning.  Before I could even turn all of the lights on, a very nervous young man handed me a urine collection cup and told me that he was dropping off a sample to the lab. 

The urine cup had a very small amount of sample and I, trying to make a joke, said, “Is that the best you could do first thing in the morning?” 

The young man sheepishly said, “yeah, sorry” and turned to walk away. 

It was only then that I read the requisition.  He was submitting a semen sample for analysis. 

Oh, will I ever learn to keep my smart assed comments to myself?!?

Haiku Friday - Week 46

May 18th, 2007

A certain cheap blue guitar picker once linked me to threadless.com because they had a certain tee shirt made just for us in the Friday Haiku Club.  It arrived this week.  

 zoom.gif

Is that not great or what?!?

You remember the rules?  5-7-5 syllables of silly bull.  What's yours? 

Posted in random | 7 Comments »

Lean, Mean, Carlene, Vegas Queen

May 13th, 2007

I have a great mom.  When I was growing up, she tried her very best to instill in me a moral set of standards that were fit for the Royal Family.  “Always tell the truth”, “sit up straight,” “take your thumb out of your mouth”, “clean your room,” she would say in her diminutive way.  My mother has always been unassuming.  In her mannerisms, her speech, even the clothes that she wears.  I picture her with her plaid skirt, matching sweater set, and a single strand of Republican pearls. 

Our house was always clean and free of clutter.  Tastefully decorated – like a picture from Better Homes and Gardens Magazine.  Mother has what I consider‘rules for a happy life’.  Do not get dirty.  Always go to church on Sunday.  It is good to tell the truth, but you do not always need to be telling it.  And when I drove home my first new car painted a very loud “pull me over red” the now famous - machines, like gloves, should be either black, white, or brown.  Mother does not go in for bold colors.  She prefers pristine white poinsettias to the rather pedestrian red color.  Red poinsettias are the “Bob Gullet” of botany.  One of my earliest memories of my mother is me strapped into my car seat,  she driving her Thunderbird (white, of course), humming along to the Dionne Warwick eight-track, tapping the steering wheel with her finger, hair meticulously coiffed into a Marlow Thomas style.  I wasn’t able to talk well, yet – but I remember thinking, “cool.”     

Mom made us strive to achieve.  She did this by subtly withholding huge amounts of meaningless praise.  She would only give just enough so as not to make our heads swell.  I could win the Nobel Peace Prize for excellent literature and she might just pat me on the back and say, “Isn’t he cute?”  She also hides her disappointment in anything that does not turn out quite the way she would wish.  When it comes to my eccentric and eclectic décor – she simply states, “Nice.”  When it came to calling off my engagement to a girl, I had dated for five years because I had to accept the fact that I am homosexual – she changed the subject, although she knew.  They always know. 

Because of my mother’s influence, I am probably the straightest looking gay man you will ever meet.  Of course, I press my clothes, but they consist of quiet colors, traditional button down plaid shirts, and sensible shoes.  I wear tweed jackets and cardigans.  I dress like a cross between Mr. Rogers and Prince Charles.  I do not go in for wild hairstyles or colors, and (except for a short stint with an earring) I do not have “body jewelry.”  I am soft spoken and mild mannered – just like mom.  I work hard at my job, and I rarely, if ever, go out to “party,” I keep a clean house and always try to do the right thing. 

Consider, then, the contradictions in my mother’s behavior, as she grows older.  After years of knowing her, in fact expecting her, to act a certain way; she is exhibiting certain questionable tendencies that worry me.  In fact, they frighten me.  My mother thinks nothing of flying to New York City with my aunt to spend a week shopping, or seeing shows, or what ever else they do in NYC.  Picture it, my mother, walking the dangerous streets of New York – by herself – with no other protection!  Once when she visited, she brought back imitation Rolex watches as gifts.  I believe these to be illegal.  She was even present during a raid of these seedy, illegitimate, booths.  She asked me if I wanted her to bring one home for me.  “No, thanks” I said, “my karma is doing pretty good right now and I’d rather not jeopardize it.”  Honestly, my mother, a common counterfeiter. 

As if this was not bad enough, both mom and dad have been visiting Las Vegas – a lot!  Several times a year, they fly out with my auntie and gamble away the inheritance.  My mom says that she and my auntie put sparkle glitter on their hands when they play.  Sparkle glitter?  My mother?!?  What in God’s name is next?  Gold lame?  When not in Las Vegas, they visit other fine gambling establishments all over the country.  I think it may be becoming serious.  Not that I object to the money spent – but the mental image of my mother in a smoke filled casino playing the slots is just too disturbing! 

This past summer, my folks came out to pay us a visit.  They had been to a gambling casino in the nearby city of St. Louis – and thought since they were out this far, why not come down and spend the day.  They left earlier than planned so that my dad could have one more crack at blackjack.  I am beginning to think an intervention is needed.  My auntie herself while canceling plans to come and stay said that she had a better offer in Atlantic City.  I have taken second fiddle to the casinos.  My mind boggles at the thought. 

It is interesting that as we grow older, so does the perception that we once had of our parents.  When we are small, our parents are authoritarians who know all.  When we are in our teens and twenties, they know nothing and are powerless, so we must rebel.  Now that I have finally reach adulthood, with all the lessons and ups and downs – I see my parents as people.  In fact, I see them as interesting people.  I guess I like that after all.   

Haiku Friday - Week 45

May 11th, 2007

Lazy, drifting, waves

Down the swelling Missouri

Sandbags anyone?

We sure are getting lots of rain here.  Everyone keeps talking about the big flood of 1993 where the entire town was under water.  Nice.  I suppose I can canoe to work.  

So anyway, you remember the rules?  5-7-5 syllables of silly bull.  What's yours? 

Posted in random | 6 Comments »

Arriving OCD

May 10th, 2007

Idiosyncrasies.  We all have them.  Those unusual behavior patterns that seem completely reasonable to us, but are enough to make someone else think we’re bat shit crazy.  As I'm getting older, I've noticed that I'm getting more rigid in my routines.  I suppose I could be labeled as OCD, but I think that’s a bit extreme.  I'm just particular.  So, you be the judge.  In the spirit of sharing, here are some random acts of peculiarity that are part of being me: 

  • Toilet paper must roll over, not under the roller.  This is Universal Law and when visiting any cubical I will change the roll to the correct aforementioned “over” position.  This includes your bathroom as well.  Consider yourself warned.
  • In my medicine cabinet, all items are arranged so that the label is clearly visible.  Additionally the bottles, on the second shelf, starting from left to right, in the order I take them each morning, you will find a multivitamin, iron tablets, zinc tablets, biotin capsules, and aspirin.  Pain relievers, cold medicine, and a sundry are located on the top shelf.  The lower shelf belongs to Mark and there is absolute chaos and disorder on that shelf.  I don’t even look at it and just keep my eyes upward. 
  • I wanted to start taking ginkgo biloba to improve memory retention, but it’s kept on Mark’s shelf so I kept forgetting it.  
  • My shower routine goes something like this; total rinse off, shampoo, rinse, conditioner, right arm, left arm, neck, chest, back, right thigh, right calf, right foot, between the toes, left thigh, left calf, left foot, between the toes, nether regions, rinse, shave right side, shave left side, wash my face, rinse out the conditioner.  There is absolutely no deviation.  Never.  Drying off is much the same.  HOWEVER, when brushing my teeth, I always start on the left side.  I’m riotous like that.
  • I usually eat lunch listening to All Things Considered on NPR.  If I have to miss a day then I’m very cross for the rest of the afternoon. 
  • When it comes to light switches, the dents in the screws MUST be completely horizontal.  Not crooked.  Not vertical  I’ve gone through my house, my office, and the entire laboratory correcting this.  I don’t understand why nobody else is bothered by crooked screw dents.  People!  This is madness! 
  • I only ever chew one piece of gum a day and that is during my drive to work in the morning. 
  • I have a pen fetish.  There is a drawer in my laundry room filled to the brim with pens.  On my desk at work, I have five pen holders and I’ve just counted 156 writing utensils.  In my lab coat pocket, I carry 5, yet if I’m not wearing my lab coat, I can never seem to find a pen. 
  • I collect sets of dishes.  Seriously, I have 10 complete sets of dishes, yet Mark and I tend to use the same two plates night after night. 
  • I already told you all about my book thing. 
  • I have a definite pattern of how I like to start my work day, and when there is an interruption to that routine, I get very irritated. 
  • Sure, it’s clutter, but its organized clutter.  Everything has a place and everything (and I DO mean everything) in its place.  If it isn’t, then I get antsy. 
  • I have an area rug in the front room and get absolutely crazy if the fringe isn’t all combed in the same direction.  I have a special way of combing it when it’s ruffled. 

I’m certain there are more, but I’ll stop now.  I’m beginning to scare myself!

Posted in remark | 6 Comments »

For Havin’ Sex

May 9th, 2007

I’m a sucker for any kind of accent.  I’ll admit it.  There’s something about the sing-song of an Indian accent, the steaminess of a Latino, the ruggedness of the East coast, the calming of British, or the sultry drawl of the South that makes my knees turn to jelly.  Like Professor Henry Higgins, I’m pretty good at guessing where your roots might be by listening to you speak for a while.  It is of interest to me how voice inflection and word usage is affected by regional areas.  Spend enough time on the east coast and sooner or later you’ll be asking for a “cahfee regulah”.  Travel north and you’ll eventually draw out certain words like “soooda”; whereas a few hundred miles south and “ya’ll can jist imagine”. 

Mark and I have certainly had many miscommunications and often he just stares at me trying to process a “foreign” word that I might use.  I especially like talking with the hospital surgeon who is from Czechoslovakia.  He once asked me to collect a culture on one of his patients when the nurse changed “da picking of da vound” (the packing of the wound). 

There’s an online test that places you geographically according to certain word usage such as the difference between “bag, sack, or poke” when hauling your groceries home.  You might know that my mother is from northern Ohio, my dad from the east, Mark is British, and I spent a good amount of time in southern Ohio closer to the Appalachians than the Lake.  Since I have a healthy diversity when it comes to verbal influences, I thought I’d try and stump it.  Turns out that I’m 60% south of the Mason Dixon line.  I don’t believe it, but it is on the internet, so it has to be true after all. 

Arlene and I were talking about this in the hematology lab the other day.  Her husband is from south middle Tennessee close to Alabama.  I’ve been corrected before; it is not “central” but “middle” Tennessee.  Needless to say, his accent is pretty thick and as cute as Christmas.  Arlene told me this story and with her permission, I’ll pass it along to you. 

Seems Arlene and her husband Ron decided to get away for a weekend in a small cabin by the lake one time when they were first married.  The owners of the cabin must have had some problems with people using bath towels for tasks other than bathing, so there was a stack of old rags on the kitchen counter with a poem written out on a piece of paper.  Arlene stowed the groceries as Ron read the poem aloud.  Arlene doesn’t exactly remember how the poem went except to say that it mentioned to “use me to wipe your windshield”, “use me to wipe your boots”, and the list went on.  And at the end of the poem listing various uses for the stack of rags, Ron read, “just use me for havin’ sex!”. 

Arlene called for a time out.  “WHAT was that last part again?!?” 

“just use me for havin’ sex!”, Ron repeated. 

Arlene couldn’t believe what she had just heard.  After asking for clarification one last time (“just use me for havin’ sex!”) she asked to see the poem. 

Ron’s accent was so thick that Arlene couldn’t understand him when he read, “just use me for heaven sakes!”

 

Posted in random | 4 Comments »

Ten

May 5th, 2007

A Cheap Blue Guitar picker has tagged me with one of those meme things.  I’m supposed to list 10 (ten?!?) interesting facts about my life.  Well, okay.  I’ll play, but I’m not going to promise that any of it will be interesting.

I spent much of my formative years in southern Ohio in the country close to the Ohio River which borders Kentucky and West Virginia.  Therefore, by definition, I am a hilljack redneck and have all the polish and sophistication of a hog in an evening gown.

I can do girly things like sew, crochet, and grow flowers.

I can do manly things like chop and stack wood, change a flat tire, fire a shotgun, and operate a circular saw.

I went to school with a kid who would eat any sort of bug you could find – dead or alive – for a nickel.  His name was Tim and he got an awful lot of my allowance. 

I put myself through college without any grant money.  It took me ten years, but I have a PhD in clinical microbiology and am still working on a second masters in virology.  I did this because my father always called me stupid and no good. 

I’ve never smoked a joint, but some friends and I did get completely wasted on likker one time.  I don’t recommend it.

I can’t throw away any book.  I still have all of my books from childhood, grade school, college, and many others.  At last count, it was well over 1000.  I have them catalogued by the Dewey Decimal System just like in a library.

I once stepped barefoot on a board with a nail sticking out.  It went clean through my foot. I didn’t cry. 

I’d like to someday live in the Smoky Mountains on a very remote tract of land.

To help make money for college, I worked for a couple of years as a chorus boy in the Player’s Theater in Columbus, Ohio.  The theater is now closed. 

Okay.  I'll give you all a bonus:  My biggest fear in life is to be marginalized, neglected, and ignored.  I reckon it's because I've been marginalized, neglected, and ignored.   

So, there you go.  I won’t tag anyone, but if you want to do it, be my guest. 

Posted in random | 6 Comments »